Antibiofilm activities of crude extract and supernatant of Actinobacteria against biofilm formed by foodborne pathogens and food spoilage bacteria

Bacteria form a biofilm that attaches the microbial population to solid surface, thus acting as barrier protect external stresses from bacterial community. In food industry, biofilms are believed be responsible for food-borne diseases and spoilages. This research was conducted characterize anti-quorum sensing (QS), anti-biofilm activity of crude extract, supernatant Actinobacteria isolates against foodborne, spoilage bacteria. is group filamentous spore-forming Gram-positive bacteria, which well recognized source novel secondary metabolites, such sensing. Two utilized in this study were recovered Indonesian marine environment previous study. Both demonstrated anti-QS on early detection assay using agar overlay method. The fermented, extracts obtained through extraction 100 % ethyl acetate further evaporation, while collected directly following fermentation. entire displayed capability an agent at concentration 20 mg.mL-1, alongside when its form. both supernatants based N-Hexanoyl-L- homoserine lactone (HHL) validation also categorized non-toxic Brine Shrimp Lethality Assay (BSLA).